Pathogenicity of mouse hepatitis virus for preimplantation mouse embryos
نویسندگان
چکیده
منابع مشابه
The Evaluation of Melatonin Effect on In-Vitro Development of Mouse Preimplantation Embryos
Purpose: Melatonin promotes in-vitro embryo development in different species. This study studied the effects of melatonin on in-vitro mouse preimplantation embryo development. Materials and Methods: Two-cell embryos were obtained from oviduct of 6-8 weeks female NMRI mice 48 hours after administration of an intra-peritoneal injection of 5 IU/ml pregnant mares’ serum gonadotrophin and subsequent...
متن کاملEffect of Li+ on preimplantation mouse embryos.
Two-cell mouse embryos were cultured in vitro for different periods in a medium in which NaCl was partially replaced by LiCl at concentrations ranging from 1 to 30 mM. The relative cell number diminished according to increasing LiCl concentrations but the onset of blastulation was not affected, thus resulting in blastulae with fewer cells than normal and with a reduced or absent inner cell mass...
متن کاملPreimplantation development of gynogenetic diploid mouse embryos.
Diploid gynogenetic mouse embryos were produced in a three-step procedure: fertilization in vitro, suppression of the 2nd polar body formation by Cytochalasin B, and microsurgical removal of the male pronucleus. The operated eggs were transplanted to the oviduct of recipient females for 72 or 96 h. The overall recovery rate was 73%, but compacted morulae and blastocysts constituted only 28.6% o...
متن کاملAdenosine triphosphate content of preimplantation mouse embryos.
The utilization of energy substrates by preimplantation mouse embryos undergoes several changes during development in vitro (Brinster, 1965a, b) but very little is known about the level of high-energy containing compounds, such as ATP at these stages. In the present study, the content ofATP in freshly collected preimplantation mouse embryos has been measured. For each estimation, fifty to 150 e...
متن کاملHeat shock memory in preimplantation mouse embryos.
To investigate the consequences of possible physiologic stress to embryos caused by in vitro fertilization procedures, we used heat shock response in preimplantation mouse embryos as a model. A heat shock "memory" was discovered that renders cleavage-stage embryos more responsive at the transcriptional level to secondary perturbation with very low doses of heat, even several cell cycles after t...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Reproduction
سال: 1985
ISSN: 1470-1626,1741-7899
DOI: 10.1530/jrf.0.0730207